TCR GAMMA, T CELL RECEPTOR GAMMA GENE REARRANGEMENT

PCR – Polymerase Chain Reaction

PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.

 

Test Performed At:

 

 

Barnes-Jewish Hospital Molecular Diagnostics Laboratory at Washington University

 

Specimen Required:

 

 

Call Barnes-Jewish Hospital Laboratory Customer Service at (314) 362-1470 for collection instructions.

 

 

 

 

SUBMIT ONLY 1 OF THE FOLLOWING SPECIMENS:

 

 

 

 

 

Blood

 

 

1 lavender-top (EDTA) or 1 yellow-top (ACD) tube.  Collect 1.0 – 2.0 mL of EDTA or ACD whole blood.  Invert several times to mix blood.  Send to lab at ambient temperature.

 

 

NOTE:

Please complete a Molecular Diagnostic Laboratory Request for DNA Studies-Oncology” and forward it with the specimen. This form is supplied by St. Louis Children’s Hospital or available at  http://pathology.wustl.edu/patientcare/moldiagnostic.php

 

 

 

 

 

Bone Marrow

 

 

CLOTTED SPECIMEN IS NOT ACCEPTABLE.  Place 1.0 – 2.0 mL of bone marrow in lavender-top (EDTA) tube.  Invert several times to mix bone marrow.  Send specimen at ambient temperature.

 

 

NOTE:

Please complete a “Molecular Diagnostic Laboratory Request for DNA Studies-Oncology” and forward it with the specimen. This form is supplied by St. Louis Children’s Hospital or available at  http://pathology.wustl.edu/patientcare/moldiagnostic.php

 

 

 

 

 

Frozen Tissue

 

 

10 mm3 of fresh frozen tissue in a sterile plastic container.  Send frozen tissue in container on dry ice directly to the lab.

 

 

NOTE:

Please complete a “Molecular Diagnostic Laboratory Request for DNA Studies-Oncology” and forward it with the specimen. This form is supplied by St. Louis Children’s Hospital or available at  http://pathology.wustl.edu/patientcare/moldiagnostic.php

 

 

 

 

 

Formalin-fixed, paraffin-embedded Tissue

 

 

Twenty, 10 micron sections of FFPE tissue in a sterile plastic tube.  Send FFPE tissue to the lab at ambient temperature.

 

 

NOTE:

Please complete a Molecular Diagnostic Laboratory Request for DNA Studies-Oncology” and forward it with the specimen. This form is supplied by St. Louis Children’s Hospital or available at  http://pathology.wustl.edu/patientcare/moldiagnostic.php

 

Laboratory Notes:

 

 

DO NOT PROCESS SPECIMENS.

 

Reference Values:

 

 

An interpretative report will be provided.

 

Day(s) Test Set Up:

 

 

Tuesday

 

Cut-off Time:

 

 

Monday at 1300

 

Turn Around Time:

 

 

2-5 days

 

CPT Code:

 

 

83907 - prior cell lysis

 

 

83912 – interpretation and report

 

 

83890 – isolation

 

 

83901 – multiplex each additional seq

 

 

83894 – gel electrophoresis

 

 

83900 – multiplex PCR 1st 2 seq

 

Care Manager Order Name:

 

 

Miscellaneous Referral Test ONCE RTN

 

KIDDOS Order Name:

 

 

TCR Gamma

 

Cerner Order Name:

 

 

TCR Gamma

 

Last Updated 06/09