PORPHOBILINOGEN, QUANTITATIVE, 24- HOUR URINE
High-Performance Liquid Chromatography (HPLC) with Fluorometric Detection Includes quantitation of coproporphyrins, uroporphyrins, and intermediate porphyrins (heptacarboxyl, hexacarboxyl, and pentacarboxyl). Includes liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination of porphobilinogen.
	Test Performed At:
		Mayo Medical Laboratories #8562
	Specimen Required:
		Submit an entire 24-hour urine collection in a urine container that has 5 grams of sodium carbonate added as preservative, supplied by St. Louis Children’s Hospital. Refrigerate specimen during collection and keep specimen protected from light. Please note collection start and stop date/times on container. Call 314-454-4161 to obtain container.
		NOTE:			Please forward a list of medications the patient is currently taking with the specimen.
		CAUTION:			PBG and porphyrins are susceptible to degradation at high temperature, at pH <7.0, and on exposure to light. Please be sure to collect specimen in a light-resistant container with preservative that has been added prior to beginning of collection.
	Laboratory Notes:
		Send 20.0 mL-50.0 mL (minimum 15.0 mL) of urine from a 24-hour urine collection in an amber 60 mL screw-capped bottle, protecting specimen from light. Send specimen refrigerated.
		NOTE:		1.	The acceptable preservative must be added to collection container at the start of collection to achieve a pH of >7.0.
				2.	DO NOT substitute sodium bicarbonate for sodium carbonate
		CAUTION:			PBG and porphyrins are susceptible to degradation at high temperature, at pH <7.0, and on exposure to light. Please be sure to send specimen with preservative that has been added prior to beginning of collection, in a light-resistant container.
	Reference Values:
		UROPORPHYRINS (octacarboxyl)				3-25 mcg/24 hours
		HEPTACARBOXYLPORPHYRINS				0-7 mcg/24 hours
		HEXACARBOXYLPORPHYRINS				0-6 mcg/24 hours
		PENTACARBOXYLPORPHYRINS				0-7 mcg/24 hours
		COPROPORPHYRINS (tetracarboxyl)
			Males:			25-150 mcg/24 hours
			Females:			8-110 mcg/24 hours
		PORPHOBILINOGEN				0.0-0.5 mg/24 hours
	Day(s) Test Set Up:
		Monday - Friday; 7:00 am.
	Cut-Off Time:
	Turn Around Time:
		4-6 days
	CPT Code:
		84110
	Care Manager Order Name:
		Miscellaneous Referral Test ONCE RTN
	KIDDOS Order Name:
		Miscellaneous Lab Test
	Cerner Order Name:
		Misc Ref SLC

Last Updated 03/09