PARANEOPLASTIC AUTOANTIBODY EVALUATION, CSF
Includes: Antineuronal Nuclear Ab, Type 1; Antineuronal Nuclear Ab, Type 2; Antineuronal Nuclear Ab, Type 3; Purkinje Cell Cytoplasmic Ab Type 1; Purkinje Cell Cytoplasmic Ab, Type 2; Purkinje Cell Cytoplasmic Ab, Type Tr Amphiphysin Ab, S; CRMP-5-IgG, S; and Anti-Glial Nuclear Ab, Type 1 Reflex tests: Paraneoplastic Autoantibody Wblot, CSF; CRMP-5-IgG Western Blot CSF; and GAD65 Ab Assay, CSF
Reporting Title: Paraneoplastic Autoantib Eval, CSF
Test Performed At:
	Mayo Medical Laboratories  #80013
	NOTE:	Include relevant clinical information, name, phone number, mailing address, and e-mail address (if applicable) of ordering physician.
Specimen Required:
	Send 4.0 mL of spinal fluid
Laboratory Notes:
	Send 4.0 mL (3.0 mL minimum) CSF refrigerated.
	NOTE:	Include relevant clinical information, name, phone number, mailing address, and e-mail address (if applicable) of ordering physician.
Reference Values:
	ANTINEURONAL NUCLEAR ANTIBODY-TYPE 1 (ANNA-1)			Negative at <1:2
	ANTINEURONAL NUCLEAR ANTIBODY-TYPE 2 (ANNA-2)			Negative at <1:2
	ANTINEURONAL NUCLEAR ANTIBODY-TYPE 3 (ANNA-3)			Negative at <1:2
	PURKINJE CELL CYTOPLASMIC ANTIBODY, TYPE 1 (PCA-1)			Negative at <1:2
	PURKINJE CELL CYTOPLASMIC ANTIBODY, TYPE 2 (PCA-2)			Negative at <1:2
	PURKINJE CELL CYTOPLASMIC ANTIODY, TYPE TR (PCA-TR)			Negative at <1:2
	AMPHIPHYSIN ANTIBODY			Negative at <1:2
	CRMP-5-IGG			Negative at <1:2
	ANTI-GLIAL/NEURONAL NUCLEAR ANTIBODY, TYPE 1 (AGNA-1)			Negative at <1:2
	NOTE:	Titers lower than 1:2 are detectable by recombinant CRMP-5 Western blot analysis. CRMP-5 Western blot analysis will be done on request on stored spinal fluid (held 4 weeks). This supplemental testing is recommended in cases of chorea, vision loss, cranial neuropathy, and myelopathy. Call the Neuroimmunology Laboratory at 1-800-533-1710 to request CRMP-5 Western blot.
	CRMP-5-IGG WESTERN BLOT				Negative (reported as positive or negative)
	PARANEOPLASTIC AUTOANTIBODY WESTERN BLOT CONFIRMATION				Negative (reported as positive or negative)
	GAD65 ANTIBODY ASSAY				<0.02 nmol/L
	Neuron-restricted patterns of IgG staining that do not fulfill criteria for the listed autoantibodies may be reported as “unclassified antineuronal IgG.” Comples patterns that include non-neuronal elements may be reported as “uniterpretable.”
Day(s) Test Set Up:
	Monday – Friday
Cut-Off Time:
Turn Around Time:
	4-9 days
CPT Code:
	86256-		AGNA-1
	86256 -		Amphiphysin
	86256 -		ANNA-1
	86256 -		ANNA-2
	86256 -		ANNA-3
	86256 -		CRMP-5-IgG
	86256 -		PCA-1
	86256 -		PCA-2
	86256 -		PCA-Tr
	84182 -		Paraneoplastic autoantibody Western blot confirmation (if appropriate)
	84182 -		CRMP-5-IgG Western blot (if appropriate)
	86341 -		GAD65 antibody confirmation (if appropriate)
Care Manager Order Name:
	Miscellaneous Referral Test ONCE RTN
KIDDOS Order Name:
	Miscellaneous Lab Test
Cerner Order Name:
	Misc Ref SLC

 Last Updated 02/08