BORDETELLA PERTUSSIS DNA DETECTION BY PCR

PCR (Polymerase Chain Reaction) is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.

NOTE:

This test does NOT routinely detect Bordetella parapertussis, which may account for approximately 5% of pertussis cases. Contact the Virology supervisor at (314)-454-6039 , or Virology Lab at (314)-454-2036 if testing for Bordetella parapertussis is required.

Test Performed At:
	St. Louis Children’s Hospital
Specimen Required:
	Collection guidelines:
	See: Collection Protocol for Bordetella Pertussis
	Submit two Nasophayngeal swabs as follows:
	Place one swab in Universal Transport Media (UTM)- PCR test is performed on this swab. Place one swab in Regan Lowe Media- media is available at no charge from the State lab by calling 573-751-3334. Specimen forwarded to MO State Lab if PCR testing is positive (epidemiological use only)
	Send specimen refrigerated
	Alternative specimen: a nasopharyngeal (or nasal) aspirate/ wash can be collected and transported in a screw-capped, sterile container within 24 hours of collection.
	Send specimen refrigerated
Laboratory Notes:
	Collection Kits available by calling St. Louis Children’s Hospital Laboratory Customer Service at (314) 454-4161 or from St. Louis Children’s Microbiology Laboratory.
Reference Values:
	Negative (Reported as Positive or Negative)
	Critical values (automatic callback): Any positive PCR result
	Every critical test result will be called automatically.
Day(s) Test Set Up:
	Monday - Saturday
Cut-Off Time:
	10:00 pm the evening prior to scheduled testing days
Turn Around Time:
	1-2 Days
CPT Code:
	87798
KIDDOS Order Name:
	PCR Bordetella Pertussis
Cerner Order Name:
	PCR BPER

Updated 10/08

Reviewed 09/09