HLA: ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), BLOOD
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Title: HLA: Enzyme-Linked Immunosorbent Assay (ELISA) Antibody Screen, Blood
	Test Performed At:
		Barnes-Jewish Hospital Laboratory
	Specimen Required:
		10 mL whole blood in a red/gray-top SST or plain, red-top tube(s). For young children, 5 ml in a red-top tube(s) is acceptable.
		NOTE:	Renal dialysis patients should always be drawn before the dialysis procedure.
	Laboratory Notes:
		DO NOT SPIN. Forward promptly at ambient temperature only to Barnes-Jewish Hospital HLA Laboratory. Do not refrigerate. Specimen must arrive within 24 hours of draw.
		NOTE:	HLA ELISA Antibody Identification consists of two results: Class I and II.
	Reference Values:
		Normal: negative. A positive result indicates the presence of IgG anti-HLA antibodies to either Class I or Class II antigens.
	Day(s) Test Set Up:
		Monday – Friday
	Cut-off Time:
	Turn Around Time:
		1 week routine; 2 days STAT (contact Barnes-Jewish HLA Lab 314.362.5322 to request STAT testing).
	CPT Code:
		HLA ELISA Class I
		86807		Serum screen for reactive antibody, standard method
		83516 x 2		Immunoassay non-infectious agent, qualitative, multiple step
		83912		Interpretation and report
		HLA ELISA Class II
		86807		Serum screen for reactive antibody, standard method
		83516 x 2		Immunoassay non-infectious agent, qualitative, multiple step
		83912		Interpretation and report
	Care Manager Order Name:
		HLA Studies ONCE RTN
	KIDDOS Order Name:
		HLA Monitoring Test
	Cerner Order Name:
		HLA Monitoring

 Last Updated 03/09