ESTROGENS, FRACTIONATED, SERUM

Includes Estradiol and Estrone

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Title:    Estrogens, E1 & E2, Fractionated, S

 

Test Performed At:

 

 

Mayo Medical Laboratories    #84230

 

Specimen Required:

 

 

Collect 3.0 mL (minimum 1.2 mL) of whole blood in a plain, red-top tube(s) or serum gel tube.

 

Laboratory Notes:

 

 

Spin, separate, and send 1.2 mL (minimum 0.6 mL) of serum in a plastic aliquot tube refrigerated.

 

 

NOTES:

1.

Serum MUST be separated from gel within 24 hours of collection.

 

 

 

2.

Will also accept EDTA or Heparin plasma

 

Reference Values:

 

 

ESTRONE (E1)

Children*

1-14 days:             Estrone levels in newborns are very elevated at birth

                                but will fall to prepubertal levels within a few days.

 

Males

Tanner Stages                    Mean Age             Reference Range

Stage I#:                                7.1                          Undetectable-16 pg/mL

      (>14 days and prepubertal)                       

Stage II:                                 11.5                        Undetectable-22 pg/mL

Stage III:                                13.6                        10-25 pg/mL

Stage IV:                                15.1                        10-46 pg/mL

Stage V:                                 18                           10-60 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs

for boys at a median age of 11.5 (+/- 2) years. For boys there is no

proven relationship between puberty onset and body weight or ethnic

origin. Progression through Tanner stages is variable. Tanner stage V

(adult) should be reached by age 18.

 

Females

Tanner Stages                    Mean Age             Reference Range

Stage I#:                                7.1                          Undetectable-29 pg/mL

       (>14 days and prepubertal)

Stage II:                                 10.5                        10-33 pg/mL

Stage III:                                11.6                        15-43 pg/mL

Stage IV:                                12.3                        16-77 pg/mL

Stage V:                                 14.5                        17-200 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs

for girls at a median age of 10.5 (+/- 2) years. There is evidence that it

may occur up to 1 year earlier in obese girls and in African-American

girls. Progression through Tanner stages is variable. Tanner stage V

(adult) should be reached by age 18.

 

*The reference ranges for children are based on the published

literature (1,2), cross-correlation of our assay with assays used to

generate the literature data and on our data for young adults.

 

Adults

Males:                                                    10-60 pg/mL

Females

      Premenopausal:                           17-200 pg/mL

      Postmenopausal:                         7-40 pg/mL

 

Conversion factor

E1:  pg/mL x 3.704 = pmol/L (molecular wt = 270)

 

ESTRADIOL (E2)

CHILDREN*

1-14 days:             Estradiol levels in newborns are very elevated at birth

                                but will fall to prepubertal levels within a few days.

 

Males

Tanner Stages                    Mean Age             Reference Range

Stage I#:                                7.1                          Undetectable-13 pg/mL

      (>14 days and prepubertal)

Stage II:                                 12.1                        Undetectable-16 pg/mL

Stage III:                                13.6                        Undetectable-26 pg/mL

Stage IV:                                15.1                        Undetectable-38 pg/mL

Stage V:                                 18                           10-40 pg/mL

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs

for boys at a median age of 11.5 (+/- 2) years. For boys there is no

proven relationship between puberty onset and body weight or ethnic

origin. Progression through Tanner stages is variable. Tanner stage V

(adult) should be reached by age 18.

 

Females

Tanner Stages                    Mean Age             Reference Range

Stage I#:                                7.1                          Undetectable-20 pg/mL

      (>14 days and prepubertal)

Stage II:                                 10.5                        Undetectable-24 pg/mL

Stage III:                                11.6                        Undetectable-60 pg/mL

Stage IV:                                12.3                        15-85 pg/mL

Stage V:                                 14.5                        15-350 pg/mL**

#Puberty onset (transition from Tanner stage I to Tanner stage II) occurs

for girls at a median age of 10.5 (+/- 2) years. There is evidence that it

may occur up to 1 year earlier in obese girls and in African-American

girls. Progression through Tanner stages is variable. Tanner stage V

(adult) should be reached by age 18.

 

*The reference ranges for children are based on the published

literature(1,2), cross-correlation of our assay with assays used to

generate the literature data and on our data for young adults.

 

ADULTS

      Males: 10-40 pg/mL

      Females

             Premenopausal: 15-350 pg/mL**

             Postmenopausal: <10 pg/mL

**E2 levels vary widely through the menstrual cycle.

 

Conversion factor

E2: pg/mL x 3.676 = pmol/L (molecular wt = 272)

 

 

Day(s) Test Set Up:

 

 

Monday-Friday; 100pm

 

Cut-off Time:

 

 

 

 

Turn Around Time:

 

 

4-6 days

 

CPT Code:

 

 

Estradiol

82670

 

 

Estrone

82679

 

Care Manager Order Name:

 

 

Miscellaneous Referral Test ONCE RTN

 

Cerner Order Name:

 

 

Misc Ref SLC

 

Last updated 3/08