CMV (CYTOMEGALOVIRUS) DNA DETECTION BY PCR, NON BLOOD
Polymerase Chain Reaction (PCR)/ DNA Probe Hybridization (PCR is used pursuant to a license agreement with Roche Molecular Systems, Inc.)
NOTE:			Assay performed using analyte-specific reagents. See “Analyte-Specific Reagents (ASR) – St. Louis Children’s Hospital Clinical Laboratory.”
Reporting Title: Cytomegalovirus PCR
	Test Performed At:
		St. Louis Children’s Hospital Virology Laboratory
	Specimen Required:
		NOTE:			Specimen source is required on test request form for processing.
		Body Fluids:			Send 1.0 ml of body fluid specimen refrigerated in a screw-capped, sterile vial(s). Maintain sterility and forward promptly. Specimens grossly contaminated with blood may inhibit the PCR and produce false-negative results.
		Specimen specific notes:
					CSF (Spinal Fluid)	Do not centrifuge spinal fluid specimens. Minimum accepted volume is 0.4 ml.
					Amniotic fluid	Viral culture to rule out CMV will be performed at no charge on all amniotic fluids submitted for CMV PCR testing.
					Ocular fluid	Any volume of ocular fluid is acceptable.
		Tissue:			Brain, Colon, Kidney, Liver, Lung, Etc…
					Send 1-3 cm^3 tissue in a screw-capped, sterile container(s) containing 1.0 – 2.0 ml of sterile saline or Universal Transport Medium (available through Virology Lab). Specimen cannot be frozen. Maintain sterility and forward promptly to lab.
	Reference Values:
		Negative
		Positive		Results will reported as positive for the presence of CMV DNA.
		Critical values (automatic callback): Any positive PCR result
		Every critical test result will be called automatically.
	Day(s) Test Set Up:
		Monday, Wednesday, Friday
	Cut-off Time:
		10:00 pm the evening PRIOR to day of testing.
	Turn Around Time:
		1-3 days
	CPT Code:
		87496
	Care Manager Order Name:
		PCR, Cytomegalovirus (CMV) ONCE RTN
	Cerner Order Name:
		PCR CMV

 Last updated 07/08