AIDS CLINICAL TRIAL UNIT (ACTU), BLOOD

Flow Cytometry

Includes a CD3, CD4, CD8 & CD4/CD8 RATIO

 

Test Performed At:

 

 

Barnes-Jewish Hospital Laboratory

 

Specimen Required:

 

 

SPECIMEN MUST ARRIVE WITHIN 28 HOURS OF DRAW.  SPECIMENS COLLECTED ON FRIDAYS AND 1 DAY BEFORE HOLIDAYS MUST ARRIVE BY 2:00 P.M.

DO NOT COLLECT SAMPLES ON SATURDAY.

 

 

 

 

 

Collect a total of 4.0 mL of whole blood in  2 lavender-top (EDTA) tube(s).  Send 2.0 mL of EDTA whole blood in each tube.  Forward promptly at ambient temperature only. 

 

Laboratory Notes:

 

 

DO NOT REFRIGERATE.  DO NOT SPIN.   Send 4.0 mL of EDTA whole blood to BJ Flow Lab.  Samples must be kept at room temperature. Minimum volume of 2.0 mL

 

Reference Values:

 

 

Normal Range:

%

Absolute numbers

 

 

 

Anti-C3 (Pan T Cells)

54-87

650-1770

 

 

 

Anti-CD4 (Helper/Inducer T Cells)

32-60

400-1175

 

 

 

Anti-CD8 (Cytotoxic/Suppressor)

13-43

145-820

 

 

 

CD4/CD8 Ratio

0.8-3.5

 

 

 

Day(s) Test Set Up:

 

 

Monday - Friday

 

Cut-Off Time:

 

 

22:00 Monday-Thursday, 16:00 on Friday and evenings before a Holiday

 

Turn Around Time:

 

 

2-3 days

 

CPT Code:

 

 

88184

 

 

 

88185

 

 

 

86359

 

 

 

86970

- cell preparation

 

Care Manager Order Name:

 

 

ACTU ONCE RTN

 

KIDDOS Order Name:

 

 

ACTU

 

Cerner Order Name:

 

 

ACTU

 

Last Updated 07/09