5-HYDROXYINDOLEACETIC ACID (5-HIAA), URINE
Reporting Title: 5-Hydroxyindoleacetic Acid, U
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
	Test Performed At:
		Mayo Medical Laboratories #9248
	Specimen Required:
		Submit an entire 24-hour urine collection in a urine container supplied by St. Louis Children’s Hospital. Refrigerate specimen during collection, and send specimen refrigerated. Call 314-454-4161 to obtain container. Please note collection start & stop times on container.
		NOTE:	Patient should not eat avocados, bananas, eggplant, pineapples, plums, tomatoes, or walnuts for a 48-hour period prior to start of collection.
	Laboratory Notes:
		Add 25 mL of 50% acetic acid as preservative at start of collection. Use 15 mL of 50% acetic acid for children <5 years old. This preservative is intended to achieve a pH of between approximately 2.0 and 4.0. See special instructions “Urine Preservatives” for multiple collections. Send 5.0 mL (minimum 1.0 ml) urine from a 24-hour urine collection. Send specimen refrigerated in a plastic, 13-mL urine tube. NO RANDOM OR SPOT COLLECTIONS ARE ACCEPTABLE.
		NOTE:	24-HOUR VOLUME IS REQUIRED ON REQUEST FORM FOR PROCESSING.
		Urine Preservative Collection Options
		IMPORTANT NOTE: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection, OR an acceptable preservative must be added at the START of the collection. Urine tests which require that the preservative be added at the START of the collection are noted by an * (asterisk).
				Ambient	No
				Refrigerated	Yes
				Frozen	Yes
				6N HCl	Yes (30 mL)
				50% Acetic Acid	Preferred
				Na2CO3	Yes (5g)
				Toluene	Yes (30mL)
				6N HNO3	Yes (15 mL)
				Boric Acid	Yes (10 g)
				Thymol	Yes (10%)
	Reference Values:
		<6 mg/24 hours
	Day(s) Test Set Up:
		Monday – Friday; 11:00 a.m.
	Cut-Off Time:
	Turn Around Time:
		3-5 days
	CPT Code:
		83497
	Care Manager Order Name:
		5-Hydroxyindoleacetic Acid (5-HIAA), 24 Hr Ur Bat ONCE RTN
	KIDDOS Order Name:
		5 Hydroxyindoleacetic Acid 24 Hour Urine Battery
	Cerner Order Name:
		24H Ur 5HIAA SLC

Last Updated 07/09